ISO 16221:2001 pdf free download.Water quality — Guidance for determination of biodegradability in the marine environment.
ISO 16221:2001 specifies five methods for determining the ultimate aerobic biodegradability of organic.
ISO/TC 147 has established International Standards for testing biodegradability of substances and waste water in the aquatic environment. All these methods, which are summarized in ISO 15462, can only be used for the determination and prediction of biodegradability in fresh water. There are, however, many cases, for example, substances used off-shore, where an urgent need exists for testing biodegradability in the marine environment. This International Standard describes biodegradation testing in marine test systems, and is based on an established OECD Guideline and the experience gained by a working group of the Oslo and Paris Commission (OSPARCOM) which has selected suitable standardized ISO methods, adopted for marine conditions and checked in a ring test.
2 Normative references
ISO 7827, Water quality — Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds — Method by analysis of dissolved organic carbon (DOC).
ISO 9439, Water quality — Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium — Carbon dioxide evolution test.
ISO 10707, Water quality — Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds — Method by analysis of biochemical oxygen demand (closed bottle test).
ISO 10708, Water quality — Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds — Determination of biochemical oxygen demand in a two-phase closed bottle test.
Use any available artificial seawater, or prepare an aqueous solution with the following composition. For 1000 ml of test medium, add to about 800 ml of solution g) 1 ml each of solutions h) through j) and make up to 1000 ml with solution g).
When artificial seawater is used as test medium, an inoculation is required to obtain sufficient biodegradative activity. Use as inoculum any material of marine origin, e.g. filtered seawater, suspension of marine sediment, bacteria from the filters of a marine aquarium. Take a suitable sample of the inoculum and add it to the artificial seawater to obtain a sufficient bacteria concentration in the test (see 6.2.1). Check the activity of the inoculated artificial seawater by means of the reference compound. For any required reduction of DOC in the inoculum and pre-adaptation, see 6.2.1.
ISO 16221:2001 download
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