EN 20-1-1992 pdf download.Wood preservatives — Determination of the protective effectiveness against Lyctus brunneus (Stephens) — Part 1: Application by surface treatment (laboratory method).
5 Test materials and apparatus
5.1 Biological material
Lyctus brunneus (Stephens), insects emerged from cultures not more than 48 h before use in the test, reared for at least two generations on non-enriched oak or no more than three generations on enriched oak.
NOTE The culturing of Lycrus brunneus requires care in order to obtain a regular supply of adults which have not already laid eggs. The culturing technique, which experience has shown to be suitable, is described in Annex B.
5.2 Products and reagents
5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase.
NOTE Paraffin wax with a setting point of 52 °C to 54 °C has been found suitable.
5.2.2 Gelatin, for sealing the relevant surfaces of specimens to be treated with solutions in which an organic solvent is the continuous phase.
5.2.3 Paste, for securing filter paper. The paste shall he starch-free, non-toxic to Lyctus and insoluble in the product under test.
NOTE Sodium carboxymcthylccllulose. food grade. has been found suitable.
5.2.4 Water, complying with grade 3 of ISO :3696.
5.2.5 Solvent or diluent, a volatile liquid that will
dissolve or dilute the preservative hut does not leave a residue in the wood at the end of the
post-treatment conditioning period that has a toxic effect on the insects.
CAUTION. Do not use benzene or other solvents which pose a health risk.
5.2.6 Peptone, prepared as an enzymatic hydrolysate of meat.
5.2.7 D(+) glucose
5.2.8 Filter paper ordinary qualfty, medium fast grade
5.2.9 Fine cloth of cotton or linen., with a mesh aperture of less than 0,3 mm.
5.3 Apparatus
5.3.1 Culturing chamber, with air circulation, controlled at (26 ± 1) °C, and at relative humidity (75 ± 5) %.
5.3.2 Conditioning chamber, well ventilated, controlled at (20 ± 2) °C and relative humidity (65 ± 5) %.
NOTE The conditioning of specimens may be carried out in the laboratory work area (see 5.3.4) provided that this has the conditions specified for the conditioning chamber (see 5.3.2).
5.3.3 Drying chamber, well ventilated, controlled at (30 ±2 ) °C.
5.3.4 Laboratory work area, well ventilated, where treatment of the test specimens is carried out.
CAUTION. It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours.
5.3.5 Testing chamber, with conditions identical to those of the culturing chamber (see 5.3.1).
5.3.6 Vacuum vessel(s), fitted with stopcocks.
5.3.7 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure of 700 Pa1.
5.3.8 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test.
5.3.9 Pipettes, of type specified in ISO 835, Part 1, Class B: graduated pipette with no waiting time. Capacity 1 ml with an accuracy of± 0,01 ml.
5.3.10 Safety equipment and protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator.
5.3.11 Test containers, suitable for holding the test specimens and of material resistant to the solvents used.
NOTE Jars of approximately 60 mm diameter and 100 mm height have been found to be suitable.
5.3.12 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g.
5.3.13 X-ray apparatus, (optional) with tungsten target and beryllium window, with voltage and current continuously variable in the ranges:
Voltage: 10 kV to 50 kV, Current: 0 mA to 15 mA.
6 Sampling
The sample of preservative shall be representative of the product to he tested. Samples shall he stored and handled in accordance with any written recommendations from the supplier.
NOTE For the sampling of preservatives from bulk supplies. the procedure given in EN 212 should be used.
7 Test specimens
7.1 Species of wood
The test shall be carried out on European oak. This shall comprise sessile oak, Quercus pet raea (Mattuschka) Lieblin, and pedunculate oak, Quercus robur Linnaeus.EN 20-1-1992 pdf download.
EN 20-1-1992 pdf download
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