BS ISO 21073:2019,Microscopes — Confocal microscopes — Optical data of fluorescence confocal microscopes for biological imaging.BS ISO 21073:2019 pdf free download.
BS ISO 21073:2019 specifies commonly used quantities regarding image performance in confocal laser scanning microscopy used for imaging of fluorescent biological specimens.
BS ISO 21073:2019 applies only to confocal single point scanners using single photon excitation.
This document is intended to provide comparable specifications of confocal microscopes by microscope manufacturers and to allow users to compare and monitor the imaging performance of their confocal Microscope.
A confocal laser scanning microscope in this document comprises a laser illumination light source, a scanning unit to deflect the excitation laser light, an objective and a detection unit consisting of tection pinhole and a photo detector.
Normative references
ISO 10934-1, Optics and optical instruments – Vocabulary for microscopy – Part 1: Light microscopy;
ISO 10934-2, Optics and optical instruments – Vocabulary for microscopy – Part 2: Advanced techniques in light microscopy;
Resolution is the capacity for imaging fine detail which is determined by the minimum spatial separation of two-point objects required for their observation as distinct objects. Various criteria have been proposed to determine the resolution, e.g. the Abbe, Rayleigh, Schuster, Houston or Sparrow criteria.
In general, the minimum resolvable distance is the most relevant quantity regarding resolution For practical reasons this document defines resolution as given in 4.12.

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